Tinatin I. Brelidze

Associate Professor of Pharmacology
Ph.D., Physiology and Biophysics
University of Miami, 2003
(202) 687-6178
tib5@georgetown.edu

Ion channels are integral membrane proteins that regulate the passage of ions through cell membranes.  Ion channels are essential for physiological function of living cells and abnormalities in ion channel function or expression pattern are often linked to inherited or acquired diseases. The research in my laboratory is focused on studies of mechanisms responsible for opening and closing of ion channels, identification of novel ion channel regulators and investigation of therapeutic potential of ion channel regulators.  In our studies we use a variety of methods, including electrophysiology, biochemistry techniques, fluorescence-based methods, and zebrafish and rodent animal models.

Selected Publications:

• Hayoz S, Tiwari PB, Piszczek G, Üren A, Brelidze TI Investigating cyclic nucleotide and cyclic dinucleotide binding to HCN channels by surface plasmon resonance. PLoS One. 12:e0185359, 2017
• Carlson, A.E., Rosenbaum, J.C., Brelidze, T.I., Klevit, R.E., Zagotta, W.N. Flavonoid regulation of HCN2 channels. J. Biol. Chem. 288(46):33136-45, 2013
• Brelidze, T.I., Gianulis, E.C., DiMaio, F., Trudeau, M.C., Zagotta, W.N. Structure of the C-terminal region of an ERG channel and functional implicationsProc. Natl. Acad. Sci. USA. 110(28):11648-53, 2013
• Carlson, AE, Brelidze, TI, Zagotta, WN. Flavonoid regulation of EAG1 channelsJ. Gen. Physiol. 141:347-358, 2013
• Brelidze TI, Carlson AE, Sankaran B, Zagotta WN. Structure of the carboxy-terminal region of a KCNH channel Nature 481:530-3,2012
• Brelidze TI, Carlson AE, Davies DR, Stewart LJ, Zagotta WN. Identifying regulators for EAG1 channels with a novel electrophysiology and tryptophan fluorescence based screen. PLoS One 5: e12523. 10.1371, 2010
• Brelidze TI, Carlson AE, Zagotta WN. Absence of direct cyclic nucleotide modulation of mEAG1 and hERG1 channels revealed with fluorescence and electrophysiological methods. J Biol Chem. 284:27989-97, 2009
• Zhang Y, Niu X, Brelidze TI, Magleby KL. Ring of negative charge in BK channels facilitates block by intracellular Mg2+ and polyamines through electrostatics. J Gen Physiol. 128:185-202, 2006
• Brelidze TI, Magleby KL. Probing the geometry of the inner vestibule of BK channels with sugars. J Gen Physiol. 126:105-21, 2005
• Brelidze TI, Magleby KL. Protons block BK channels by competitive inhibition with K+ and contribute to the limits of unitary currents at high voltages. J Gen Physiol. 123:305-19, 2004
• Brelidze TI, Niu X, Magleby KL. A ring of eight conserved negatively charged amino acids doubles the conductance of BK channels and prevents inward rectification. Proc Natl Acad Sci U S A 100:9017-22, 2003
• Mrevlishvili GM, Kankia BI, Mdzinarashvili TJ, Brelidze TI, Khvedelidze MM, Metreveli NO, Razmadze GZ. Liposome-DNA interaction: microcalorimetric study. Chem Phys Lipids 94:139-43,1998

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